Plasmids can be utilized in gene therapy, genetic vaccination, and DNA recombination technology. Plasmids are known as replicons, a unit of DNA that is capable of replicating autonomously by an appropriate host. ĭNA plasmids may be genetically altered and utilized in the recombinant DNA technology. One-dimensional based sensors such as carbon nanotubes and nanowires are being utilized for immediate identification of various bio-molecules with high sensitivity. Currently, various sensors such as carbon nanotube, and silicon nanowire field effect transistors (FETs) are showing high sensitivity. Surface plasmon resonance method achieved high sensitivity for label free biological detection. Various studies are aiming to design label free sensors to detect the target molecule, where a number of transduction techniques are being tested by researchers. But this method requires the biomolecule to be labeled with one enzyme for steric reasons. Moreover, self-powered temperature sensor based on Seebeck effect transduction can open the door for new methods of clinical diagnosis. PEC sensors are highly selective, however they are costly. Photoelectrochemical (PEC) technique is a new detection method, which has the electrochemical and optical techniques advantage. Health monitoring and biological detection are vital. Traditional detection methods of bio-molecules require labels which provide high sensitivity, but these methods do not provide real time detection and they are costly. Immediate bio-molecule detection is highly critical in several areas such as health and food analysis toxicity identification, and cancer detection. The results indicate a promising sensor for real-time, reliable, and cost-effective DNA detection. Moreover, increasing the concentration of DNA results in an increment in the current signal thus the response is proportional to DNA concentration. Both experimental and simulation agree where the current signal is higher for sensors decorated with trimetallic nanoclusters, which indicate higher sensitivity.
The developed sensor is investigated by both simulation and experiment.
Moreover, the sensor sensitivity is enhanced by decorating the graphite oxide channel with composite trimetallic nanoclusters that include gold, silver, and platinum. This sensor consists of two gold electrodes connected through a channel of graphite oxide. In this article, a graphite oxide FET based sensor is designed, fabricated, and characterized for real-time detection of various concentrations of nucleic acid with a detection limit of 1.28 nM of DNA. Field effect transistor (FET) based sensors are witnessing robust evolutions in the fabrication and characterization for the purpose of biomedical applications.
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